NMR Summer Workshop for Beginners

(Last update 2 June, 2008)

This Workshop is designed for undergraduate students with little NMR knowledge. Advanced students could download and read directly the Web references themselves.

Please note that all time and locations are tentative, please check for updates regularly.

Day One: Monday 11 June

         Lecture 1: NMR Instrumentation (Meng Wah S714 10:00 am - SZE)

                Demonstration of NMR Spectrometer Simulator Program MestRe-S

    Practical 1a:  Seeing a modern high field FT NMR spectrometer and

                Setting up a NMR Experiment (locking, tuning & shimming)

                (Chemistry G04 NMR Lab 11:00 am - Kit/Jennifer)

         Lecture 2: What is Multi-dimensional NMR Experiment (Chemistry P1 2:00 pm - SZE)

         Web reference: http://www.cis.rit.edu/htbooks/nmr/inside.htm

    Practical 1b:  Seeing a modern high field FT NMR spectrometer and

                Setting up a NMR Experiment (locking, tuning & shimming)

                (Chemistry G04 NMR Lab 3:00 pm - Kit/Jennifer)

        

Day Two: Tuesday 12 June

         Lecture 3: Running NMR Experiment I (Running-NMR slices 1-11) (Meng Wah S714 10 am - Jennifer)

                Demonstration of NMR Spectrometer Simulator Program MestRe-S

         Practical 2a: Calibrations: solvent (water) peak position, 90 degree pulse width

                (Chemistry G04 NMR Lab 11:00 am - Kit/Jennifer)

         Lecture 4: Running NMR Experiment II (Running-NMR slices 11-20) (Chemistry P1 2:00 pm - Jennifer)

         Practical 2b: Calibrations: solvent (water) peak position, 90 degree pulse width

                (Chemistry G04 NMR Lab 3:00 pm - Kit/Jennifer)

Day Three: Wednesday 13 June

         Lecture 5: Processing NMR data (Chemistry P1 2:00 pm - Yinhua)

                Demonstration of NMR Processing Program MestRe-C

         Practical 3: Data processing and plotting (Chemistry Library 3:00 pm - Lawrence/Yinhua)

                Practice on NMR Processing Program MestRe-C

Day Four: Thursday 14 June

         Lecture 6: Protein NMR (Meng Wah S714 10:00 am - Kit)

         Web reference: http://daffy.uah.edu/nmr/BioNMR_Basics.pdf

                Demonstration of NMR Anaysis Program Sparky

         Practical 4: Spectral Analysis (Chemistry Library 11:00 am - Lawrence/Yinhua)

                Practice on NMR Anaysis Program Sparky

         Lecture 7: COSY & TOCSY Experiments (NMR-chem slices 1-13) (Chemistry P1 2:00 pm - Yinhua)

         Web reference: http://www.chem.queensu.ca/FACILITIES/NMR/nmr/webcourse/

         Practical 5: Data processing and plotting & Spectral Analysis 

                (Chemistry Library 3:00 pm - Lawrence/Yinhua)

                Practice on NMR Programs MestRe-C & Sparky

Day Five: Friday 15 June

         Lecture 8: NOESY & ROESY Experiments (NMR-chem slices 14-26) (Meng Wah S714 10:00 am - Yinhua)

         Web reference: http://www.chem.queensu.ca/FACILITIES/NMR/nmr/webcourse/

         Lecture 9: Loloatins (Meng Wah S714 11:00 am - Lawrence)

Software/Manual Can be Installed in your Own PC:

    TightVNC: http://www.tightvnc.com/

    NMR Tutor: http://members.aol.com/charlieabr/downloads.html

    Bruker NMR Spectrometer Working Guide

    NMR processing & Analysis software: MestRe-C http://www.mestrec.com/MestRe-C.html

    NMR Spectrometer Simulator: MestRe-S http://www.mestrec.com/MestRe-S.html

    Multidimensioal NMR Data Analysis: Sparky http://www.cgl.ucsf.edu/home/sparky/

Here is some useful Reviews, books and web sites:

Web sites:
NMR Basics eBook: http://www.cis.rit.edu/htbooks/nmr/inside.htm
Biomolecular NMR Workshop: http://daffy.uah.edu/nmr/workshop.html
Advanced NMR Spectroscopy: http://208.7.154.206/gmoyna/NMR_lectures/NMR_lectures.html

Reviews:
Wuthrich K. (2003) NMR studies of structure and function of biological
macromolecules (Nobel Lecture) J. Biomol. NMR. 27 13-39.
Nature Structural Biology, 1998, Volumn 5, Issue 7, NMR Supplement

Books:
NMR of proteins / edited by G.M. Clore, A.M. Gronenborn.
Boca Raton : CRC Press, 1993.

Protein NMR Spectroscopy: Principles and Practice / edited by J. Cavanagh,
W.J. Fairbrother, A.G. Palmer III, N.J. Skelton, Academic Press, 1996.

NMR of macromolecules : a practical approach / edited by Gordon C.K.
Roberts. Oxford : IRL Press at Oxford University Press, c1993.

NMR data processing / Jeffrey C. Hoch, Alan S. Stern.
New York : Wiley-Liss, c1996.

NMR Spectroscopy and its Application to Biomedical Research / S.K. Sarkar.
Netherlands: Elsevier, c1996. (Chapter 5: Dynamics studies and Model Free
Approach)

UNIX:
 Introduction: http://www.rice.edu/IT/help/documents/unix/unix01.pdf
Unix Command Reference Card: http://www.rice.edu/IT/help/documents/unix/unix01.01.pdf
General Web Resources: UNIX universe