Biophysicals and Fundamental
radicals are believed to play important roles in various biological
processes, including aging and Parkinson’s disease but they are
understood quite poorly at present. In our laboratory, we employ
mass spectrometry and computational models to study the energetics
of and pathways toward the formation and dissociation of peptide
radical ions. Through our investigations, we seek a better
understanding of the methods of generation of peptide radicals, as
well as new insights into the fundamental aspects of their
biophysical chemistry, such as the mechanisms of their gas phase
radical ion fragmentations.
Bioanalyticals and Next Generation
Mass Spectrometry Instrumentation:
We are actively
engaged in the development of new instrumentation and techniques for
the bioanalytical applications of liquid chromatography/mass
spectrometry. In collaboration with MDS-SCIEX and IONICS (Canada), we modify existing
mass spectrometric hardware to explore new avenues for proteomic,
glycomic, and metabolomic analyses. Our goals are to develop
sensitive, efficient, and novel mass spectrometric methodologies.
Mass Spectrometry-Based Proteomics,
Glycomics and Metabolomics:
research in our group focuses on the applications of mass
spectrometry to proteomics, glycomics, and metabolomics. In
collaborations with biologists, biochemists, and obstetricians
within the faculties of Science and Medicine in the University and
across the world, we seek to identify and characterize proteins, phosphoproteins,
glycoproteins and nitrated proteins that participate in several novel and important
cellular functions. Using our analytical tools, we also study
metabolomics with the goal of identifying metabolites of great
botanical and biomedical interest.
Analysis of Toxic Organic Substances in Food Matrixes
The presence of toxic organic substances such as pesticides, naturally occur toxins, drug residues and artificial chemicals additives in food may cause severe short term or chronic health problems. There are huge un-matched demands in providing more sensitive and efficient analysis for food safety testing to better protect the general public and to reduce the trade barriers in food items over the world. We are interested in coupling Multi-Dimension Liquid Chromatography to mass spectrometry with dual ESI and APCI interfaces in attempts to tackle some of the challenging issues in food analysis such as matrix interferences, insufficient sensitivity for low level toxic substances and related metabolites, and lengthy sample preparation prior to LC-MS-MS analysis.
analysis of endothelial cells
B: A novel metabolic
gene in sorghum and zebrafish embryos;
C: MRI image of DDD
(Degenerative Disc Disease);
D: MRI image of Macaca
brain (Degenerative disorder)
1. Quan Q, Song T, Hao Q, Siu CK, Chu IK. J. Am. Soc. Mass Spectrom. 2013, 24 (4), 554-562.
2. Kong RPW, Quan Q, Hao Q, Lai CK, Siu CK, Chu IK. J. Am. Soc. Mass Spectrom. 2012, 23(12), 2094-2101.
3. Hao Q, Song T, Ng DCM, Quan Q, Siu CK, Chu IK. J. Phys. Chem. B. 2012, 116 (26), 7627-7634.
4. Song T, Hao Q, Law CH, Siu CK, Chu IK. J. Am. Soc. Mass Spectrom. 2012, 23(2), 264-273.
5. Kong PW, Siu SO, Lee SMY, Lo C, Chu IK. J. Chromatogr. A 2011, 1218(23), 3681-3688 .
6. Siu SO, Lam MPY, Lau E, Kong PW, Zhang J, Lee SMY, Chu IK. Proteomics. 2011, 11(11), 2308-2319.
7. Laskin J, Yang ZB, Song T, Lam CNW, Chu IK. J. Am, Chem. Soc. 2010, 132(45), 16006-16016 .
8. Laskin J, Yang Z, Chu IK. J. Am. Chem. Soc. 2008, 130, 3218-3230.
9. Chu IK, Zhao J, Xu M, Siu SO, Hopkinson AC, Siu KWM. J. Am. Chem. Soc. 2008, 130, 7862-7872.
10. Laskin J, Futrell JH, Chu IK. J. Am. Chem. Soc. 2007, 129, 9598-9599.
11. Laskin J, Yang ZB, Lam C, Chu IK. Anal. Chem. 2007, 79, 6607-6614.